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KMID : 0363620060270030088
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Journal of Korean Oriental Medicine
2006 Volume.27 No. 3 p.88 ~ p.106
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Immunological Study of the Gami-sopunghwalhyeol-tang (Ji{={a}}w{`{e}}i-sh{={u}}f{={e}}nghu{`{o}}xu{`{e}}-tang: GSHT) on Rheumatoid Arthritis in Human Fibroblast-like Synoviocytes
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Kim Tae-Young
Song Young-Il
Yoon Il-Ji
Oh Min-Seok
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Abstract
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Objectives: This study was carried out to find the immune responses of the Gami-sopunghwalhyeol-tang (Ji{={a}}w{`{e}}i-sh{={u}}f{={e}}nghu{’’{o}}xu{`{e}}-tang) (hereinafter referred to GSHT) to the human fibroblast-like synoviocytes (hFLSs) isolated from patients with rheumatoid arthritis.
Methods: Experiments were performed to measure the cytotoxity against hFCs and the production of pro-inflammatory cytokines in hFLSs and the production of NO, ROS.
Results: 1. The gene expression of TNF-a, IL-6, IL-8 in hFLSs was effectively reduced at 100{mu}g/ml, whereas IL-1 beta was effectively reduced at 100 and 10{mu}g/ml of GSHT. 2. The gene expression of ICAM-1, MMP-3 in hFLSs was effectively inhibited at 100 and 10{mu}g/ml of GSHT, whereas TIMP-1 was effectively increased at 100 and 10{mu}g/ml of GSHT. 3. The gene expression of NOS-II in hFLSs was effectively inhibited at 100{mu}g/ml of GSHT. 4. The production of NO and ROS in hFLSs was inhibited at 100 and 10{mu}g/ml of GSHT. 5. The proliferation of hFLSs was significantly inhibited at 100{mu}g/ml of GSHT.
Conclusions: Comparison of the results for this study showed that Gami-sopunghwalhyeol-tang (Ji{={a}}w{`{e}}i-sh{={u}}f{={e}}nghu{’’{o}}xu{`{e}}-tang: GSHT) had immunomodulatory effects of suppressing or enhancing.
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KEYWORD
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Gami-sopunghwalhyeol-tang (Ji{={a}}w{`{e}i-sh{={u}}f{={e}}nghu{’{o}}xu{`{e}}-tang):rheumatoid arthritis, immunomodulatory
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